Goethe University Frankfurt, positioned among the top international research universities, offers a wide variety of academic programmes, a diverse group of research institutes, and a focus on interdisciplinary approaches to solving complex problems. The university is named after Johann Wolfgang von Goethe, the Frankfurt-born polymath renowned for his exceptional contributions to literature, science, and philosophy. Founded in 1914 with private funding and inspired by the legacy of the European Enlightenment, Goethe University stands out as a pioneering “citizens’ university”—and the history of the university is one of openness and public participation. Today, Goethe University is one of the only universities in Germany that enjoys significant public funding alongside administrative autonomy and the ability to create a private endowment.
UFRA has expertise in the structural analysis of RND transporters via X-ray crystallography.
Role: Structural analysis via X-ray crystallography of the RND components of E. coli, P. aeruginosa, E. aerogenes, K. pneumoniae, S. typhimurium and C. jejuni in complex with substrates and inhibitors.
Key facilities and infrastructure: biochemical and structural analysis of membrane proteins, particularly RND inner membrane components of tripartite multidrug efflux transporters. Main structural technique is X-ray crystallography and biochemical analysis (substrate transport, substrate binding) and HT cloning and amplified expression of membrane proteins. The group has instrumentations geared towards HT cloning, expression, purification and crystallization of membrane proteins (Genomic robotic station, crystallization robotics and plate hotels for automatic documentation and analysis of crystallization trials. Access to in-house and synchrotron Xray sources is available on a regular basis.