The team participates to the Institute of Structural Biology and Microbiology (Federative Institute of Research, IFR88). We recently develop an automatic platform for the screening of antibacterial molecules and used this platform for testing various chemical libraries. In addition, we have access to several campus technical platforms (genomic and proteomic). The team organizes a strong interdisciplinary teaching program for master students in structural biology, molecular biology and microbiology (focused on bacterial transport and antibiotic resistance).
Role: Selection and study of documented (susceptible and resistant) Gram-negative bacterial strains, Biological determinations of antimicrobial activities, Regulation and expression of membranes transporters (porins, efflux components), QSAR, chemical synthesis and characterizations of original compounds as efflux pump blockers, provide purified clinical relevant porins, synthesis of chemical compounds as inhibitor for the efflux pumps.
Key facilities and infrastructure: UMR-MD1 has an expertise in (i) membrane permeability and regulation of bacterial membrane proteins, including genetic and molecular aspects of antibiotic transport (uptake and efflux) and inhibitors of efflux pumps (special concerns with antibiotic susceptibility and resistance in Escherichia, Enterobacter, Klebsiella and Providencia); (ii) functional genomics of bacterial pathogens including Escherichia coli, Enterobacter aerogenes and Klebsiella pneumoniae, including expression, purification and reconstitution of pore-forming proteins; (iii) design and chemical synthesis of inhibitors of drug efflux transporters. The group has facilities for microbiological-biochemical laboratories, genomics, membrane protein expression and purification, for chemical laboratories (synthetic chemistry), and automated platforms for screening antibacterial compounds, and shares facilities for microbial safety laboratories, proteomics and structural analyses and membrane protein reconstitution analyses.